Composite

Part:BBa_K1893016:Design

Designed by: Henry Lloyd-Laney, Stefan Grossfurthner   Group: iGEM16_Imperial_College   (2016-10-14)


Arabinose inducible gp2 (pBAD+gp2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1342
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1473
    Illegal SapI site found at 1098


Design Notes

Gp2 was characterised using an arabinose inducible promoter, because constitutive expression, and the resultant arrest of E. coli growth, would result in several potential problems in the cloning and characterisation workflow. The sequence for Gp2 was obtained from the NCBI, and codon optimisation was not required, as the protein is from en E. coli phage.

Source

Gp2 was synthesised with a ribosome binding site using the ThermoFisher GeneArt service. This basic part (BBa_K1893019) was ligated in front of our pBAD construct BBa_K1893015

References